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Exp Content Document Name: tumor necrosis factor-related apoptosis-inducing ligand in primary biliary cirrhosis of the liver injury Introduction: This study was to investigate the tumor necrosis factor-related apoptosis inducing ligand in primary biliary cirrhosis of liver injury, from a new angle reveals the pathology of primary biliary cirrhosis mechanism. First, create your own real-time application of quantitative RT-PCR method to detect patients with primary biliary cirrhosis TRAIL in peripheral blood mononuclear cells gene expression; and then were treated with flow cytometry and ELISA detection of membrane-type surgery and can be Soluble TRAIL protein, TRAIL and analysis of primary biliary cirrhosis reflects the extent of -glutamyl transferase and alkaline phosphatase relevance. Then, normal human liver cell culture in vitro with different concentrations of TRAIL protein and / or bile role in the different time points, application MTT cell proliferation was observed on the impact of, PI staining and cell cycle, DNA content, Annexin V / PI early and late apoptotic cells detected by fluorescence staining of mitochondrial damage, immunocytochemical staining of bcl-2 molecule expression. The study found that patients with primary biliary cirrhosis TRAIL gene and protein expression were significantly higher, this abnormal expression of primary biliary cirrhosis and the occurrence and severity are closely related. Recombinant human TRAIL protein on apoptosis of normal


human liver cells, and there is concentration and time dependent; bile can promote their apoptosis. Mitochondrial damage and the expression of bcl-2 molecules may be involved in TRAIL and / or bile induced injury in normal human liver cells.
This study was designed to explore the role of TNF-related apoptosis inducing ligand in liver injury of primary biliary cirrhosis, and reveal the new pathomechanism of PBC. Based on TaqMan-MGB probe technology, real-time fluorescent quantitative RT-PCR was set up and used to detect the TRAIL mRNA level in periphery blood mononuclear cells (PBMCs) from PBC patients. Meanwhile, the TRAIL level on PBMCs was determined by flow cytometry and the plasma level of sTRAIL was measured with ELISA. In addition, the ass . Document Name Tumor necrosis factor-related apoptosis-inducing ligand in primary biliary cirrhosis of the liver injury Article Name
English (English) Translation Study on Role of TNF-related Apoptosis Inducing Ligand in Liver Injury of Primary Biliary Cirrhosis; of Liang Yan; Instructor: Jones Lang in front of people; Xian-Tao; Han Huanxing; Tan Longyi; Dengan Mei;
Agencies, Second Military Medical University; Document
Article From Shanghai Institute of Metallurgy; Materials Physics and Chemistry (Professional) PhD thesis in 2000 Keywords Primary biliary cirrhosis; tumor necrosis factor-related apoptosis-inducing ligand; real-time quantitative reverse transcription - polymerase chain reaction; tumor necrosis factor; flow cytometry; enzyme-linked immunosorbent assay technique; cell proliferation; cell cycle; apoptosis; mitochondria; Keywordsprimary biliary cirrhosis; TNF-related apoptosis inducing ligand; real-time fluorescence quantitative RT-PCR; Tumor necrosis factor; Flow cytometry; ELISA; Proliferation; Cell cycle; Apoptosis; Mitochondria;
Your location: cirrhosis "Symptoms associated with primary biliary cirrhosis edit this page collection of knowledge: gxbcom Add Date : REprimary biliary cirrhosis ( PBC) is a progressive destruction of intrahepatic bile ducts due to chronic cholestasis and to the main feature I want to transplant because of disease of unknown tags: primary biliary cirrhosisPrimary biliary cirrhosis and other autoimmune diseases, such as regular rheumatoid joint reclass syndrome scleroderma coexisting with chronic lymphocytic thyroiditis and other more common in middle-aged women after a slow and insidious onset with mild symptoms early by not Patients generally prescribe circumstances where the fetus was no more good appetite and body weight decreased about% of that treated patients may have no symptoms so the doctor of individuals with chronic unexplained sexual tension in patients with obstructive jaundice, especially verbal abuse should be accompanied by detailed understanding of steatorrhea onset disease plus incentive and team progress are there any other autoimmune diseases, attention and secondary biliary cirrhosis and other cirrhosis of the liver causes jaundice abdominal orthopedic be identifiedPrimary biliary cirrhosis and experts need to be differentiated from other diseases, including: the complexity of extrahepatic biliary obstruction with chronic active hepatitis to primary sclerosing cholangitis, drug-induced cholestasis not possible to stabilize a cure as soon as possible extrahepatic biliary obstruction should exclude Ultrasound is easy and under certain circumstances ERCP is unclear benevolence is also necessary to check liver biopsy can be noble but often non-specific diagnosis of a small number of cases to be grateful for disease diagnostic laparotomyThe disease often associated with other autoimmune diseases such as rheumatoid joint class, Sjogren's syndrome, scleroderma, chronic lymphocytic thyroiditis and other co-exist, more common in middle-aged women, insidious onset, after a slow, early symptoms of mild Patients generally in good condition, no significant loss of appetite and weight declined more than about 10% of patients may have no symptoms.
Of unexplained chronic progressive obstructive jaundice patients, especially those associated with steatorrhea, the incentive should learn more about the onset and progression of the situation, whether the existence of other autoimmune diseases, attention and secondary biliary cirrhosis and Other reasons cirrhosis jaundice were identified.




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