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August 11, 2010 . Key words retinal hemorrhage; age-related macular changes
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August 11, 2010 . Key words retinal hemorrhage; age-related macular degeneration; intravitreal injection technique. Clinical Ophthalmology, 2] . hidden directory: Documents Tags: Restructuring Man Group
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Abstract: Objective To observe the age-related macular degeneration (AMD) choroidal neovascularization (CNV) of indocyanine green angiography (ICGA) Histopathological changes in the performance of their relationship. Methods ICGA of AMD diagnosed 21 patients with 21 eyes, the CNV under the ICGA findings into active phase, regression phase, stationary phase, removed by vitrectomy CNV, optical microscopy, transmission electron microscopy of the CNV of different histopathological characteristics. The results of active CNV9 eyes, histopathological characteristics of the large number of CNV, no pigmented cells and fibrous tissue or a small amount of pigment cells in a portion of its parcel; regression of CNV9 eyes, histopathological characteristics of the active CNV compared with less more pigment cells, a small amount of fibrous tissue; quiescent CNV3 eyes, histopathological characteristics of a large number of fibrous tissue, there is little or no CNV CNV, non-pigmented cells. Conclusion CNV of exudative AMD histopathological characteristics of patients with ICGA performance has some relevance. Number of pages: 4 page range :71-74 Key words: choroidal neovascularization in age-related macular degeneration histopathologic diagnosis of indocyanine green angiography subject classification: R774.5 [medicine, HEALTH "Eye SCIENCES" the retina and optic nerve dise macula, the central fossa disease] R773.4 [medicine, HEALTH "Eye SCIENCES" eye pigment layer (uvea) dis choroidal diseases] Related Articles: Topics related references (3) coupling the literature (1)
Sulfur dioxide poisoning, pigmented choroidal retinal vein next to the shrinking 3 Kampik A, Kenyon KR, Michels RG, et al.Epiretinal and vitreous membranes.Comparative study of 56 cases J. Arch Ophthalmol, 1981; 99:1445 5 Sought-mail: 61663282@qq.com
Sanjiu Paper Network is committed to writing and presented papers for you to supply an amateur act, with more than 200 national, provincial, Focus magazine, collaborative, multi-year paper issued by the experience, with rich media capital of the French published articles on the right track, peace, too fast, and efficient. Heidelberg, Germany, using confocal laser scanning system (HRA), the 25 mg indocyanine green (Liaoyang Third Pharmaceutical Factory produces) ablation with injection of water into a 3 ml solution. Take a large number of solutions to stop enrichment, in the elbow before the intravenous injection of 1 ml diluent for allergy test. If no allergic reaction, within the elbow in the 5 s before the intravenous injection too fast will end. While launching synchronization timer, image monitoring and recording system in the image on the monitor look ICGA images, remove images extractor for elucidation of the image system, and prepared for later disposal.
Using SAS software, data processing to take rank and examination.
Abstract Objective To discuss age-related macular degeneration (AMD) clue vascular regeneration membrane (CNV) in pathological changes in characteristics. Ways to select 22 cases of AMD patients with CNV membrane, under light microscopy and transmission electron microscopy in the elucidation of CNV membrane preparations rebirth of the number of blood vessels, childish level of pigment cells (xibao) package, how many of fibrous tissue. Results were indocyanine green angiography (ICGA) stage, the invention of the CNV membrane deeds: see a large number of CNV, colorless naturally cells (xibao) and fibrous tissue or a large number of pigmented cells; vascular endothelial cells thin, on behalf of the Xiexing Wang, organelles rich, immature blood vessels. Recession CNV membranes: CNV than deeds of the cut, there are more pigmented cells, a large number of fibrous tissue, vascular endothelial organelles, cytoplasm than the deeds of the cut than the majority of the vessel lumen. Movement of the CNV membrane: see a large number of fibrous tissue, without CNV, or a little CNV, naturally colorless cells; rare vascular endothelial cells, see a large number of collagen fibers. ICGA CNV in AMD judge differences in the staging of the pathological changes in the organizational coherence. Key words age-related macular degeneration; clue membrane regeneration vessel; pathological changes
This seminar, the deeds of the rebirth of CNV with a large number of blood vessels, without RPE, fibrous tissue wrapping; electron microscope invented most of the blood vessels are immature, so angiography, ICG dye is easy to leak. Recession of the rebirth of CNV with a large number of blood vessels, surrounded by a large number of pigmented cells in outflanking, and see fibrous tissue, ICG and plasma albumen National Cheng Kung University, Department of Molecular helpless even penetrate the pigment cells, fibrous tissue wrapped, so the apparent contrast in a circle around the CNV low fluorescence. CNV can be seen more macrophages, a large number of lymphocytes present, tissue bleeding have been affirmed, grinding, going backwards. There is very little movement of the rebirth of CNV blood vessels, pigment cells, containing high-density fibrous tissue, no leakage of ICG dye, ICG dye is less than her inability to penetrate the high-density fibrous tissue of the package. Electron microscopy: a rare vascular endothelial cells, a large number of collagen fibers surrounded the blood vessels around the meticulous and has a rare macrophages, lymphocytes, pigment cells, so the disease tends to exercise. Therefore, differences in growth of CNV membranes during the course of the composition of each membrane, how many of each component, distribute different, first and foremost dependent on the rebirth of the number of blood vessels, naive level, pigment cells of the parcel, how many of fibrous tissue pathological changes in the organization characteristics.
2 Results
The seminar followed the invention to the CNV regressed pause duration, gradual reduction of vascular regeneration. Vascular endothelial cells from thin, on behalf of the Xiexing Wang, lumen small, thin basement membrane, to the thin flat endothelial cells, metabolism landing lumen widened basement membrane layer, thickening, scarring the initial CNV membrane fibers.
Age-related macular degeneration (AMD) is divided into atrophic and exudative, exudative AMD, the result is a loss as to how the growth of vascular regeneration membrane (CNV) of the composition. CNV of exudative AMD is the main reason for improved eyesight. CNV diagnosis and staging of the plain is the treatment of exudative AMD moment. At the present there is no country on the same scale CNV classificati. We discuss later in accordance with exudative AMD in the clue membrane indocyanine green angiography (ICGA) and histopathological characteristics of the CNV is divided into deeds, recession and the movement of 2. Further demonstrate their deeds in this discussion period, a period of recession and the movement of the CNV histopathology and ultrastructural characteristics of the generation of CNV, growth and deep knowledge of outcome. ICGA raid invention, deeds of CNV 9 eyes, early, early CNV strong fluorescence are visible; recession CNV9 eyes, CNV strong fluorescence only in the early present or only present at an early stage and no fluorescein leakage than the ; movement of the CNV 4 eyes, only in the early scar false fluorescence. Elucidation of light microscopy and transmission electron microscopy in control CNV membranes of cells and extracellular matrix (ECM). CNV membrane with vascular endothelial cells (VEC), pericytes, pigment epithelium (RPE), pigment cells, collagen fibers, macrophages, monocytes, lymphocytes, fibroblasts, sequential order, cut, detailed in each specimens in the content, distribution in Table 1. Table 1 CNV membranes of cells and ECM (abbreviated)
Deeds of the CNV membrane: thin endothelial cells, on behalf of the Xiexing Wang, organelles (mitochondria, rough endoplasmic reticulum) fruitful, lumen small, most of the blood vessels are immature. Rebirth scattered next to the vessel lumen, a large number of pigment cells, pigment cells contain pigment granules, pigment cell membrane without microvilli. CNV seen in thin basement membrane, scattered in a large number of pigment cells, occasional macrophages. Recession CNV: vascular endothelial organelles, the cytoplasm of the CNV membrane cut more business deals, compared with the majority of the vessel lumen. Vascular endothelial cells have fenestrae, showing that more pigment cells, cells without normal shape, see the round, spindle, etc., how many contain pigments ranging from immature tight junctions between cells without microvilli. CNV can be seen more macrophages, and some macrophages had engulfed pigment, a large number of lymphocytes, white blood cells. Cells shows a large number of collagen fibers. Movement of the CNV: a rare vascular endothelial cells, flat shape, cytoplasm, sparse organelles have been more significant, and the basement membrane layer thickening, see a large number of collagen fibers surrounded the blood vessels around the careful, shown in Figure 1 Data and methods
1.2 way
2 Yaping, Li Xiaoxin, Zhang Xiaoguang, et al. Exudative age-related macular degeneration clue membrane regeneration of blood vessels indocyanine green angiography and pathological changes of the related discussion J. Ocular Fundus Diseases, 2004; 20 (2): 69 7 22 patients with CNV membrane preparations in 10% neutral formalin flow, ash-embedded, sliced, HE staining, light microscopy stop look, and identification of cells with reference to the literature 3,4. After 1% osmium tetroxide flow, slicing, uranyl acetate lead citrate and epoxy stained with electron microscope JEM 100 Stops. CD68 identifies a token flag macrophage immunohistochemistry.
Deeds of the CNV membrane: see a large number of regeneration vessel ( ), no RPE cells, pigment cells and fibrous tissue or a large number of pigment cells () scattered distributed in the CNV or in the CNV sector outflank, and the recession, the movement of the match significantly different (P 0.05).March 5, 2010
1 Masami Nakajima, Hiroyuki Shimada, Misao Sato, et al.Indocyanine green angiography and histopathology of choroidal neovascular membrane in age related macular degeneration J. Jpn J Ophthalmol, 2000; 44:360 1.2.1 ICGA raid mode
2001 to 2008, our hospital diagnosed 22 patients with exudative AMD, 22 eyes, Spring 54 ~ 81 (even 67.9) years; male 16, female 6 cases. Eyesight scale: index / 30 cm ~ 0.4, underwent ICGA CNV membrane phases will be confiscated: deeds of 9 cases; recession, 9 cases; movement of the 4 patients underwent vitrectomy with subretinal CNV membranes out surgery. CNV membranes obtained intraoperative pathological specimens to stop raids.
4 Green WR, Retina.In: Spencer WH, ed.Ophthalmic pathology: an aatlas and text.3rd ed.Philadelphia: WB Sauders, 1985;.
2.3 CNV membrane TEM
CNV membrane is constructed rebirth blood vessels, cells and matrix three departments. In addition to VEC cells and pericytes components, the first for the pigment cells and fibroblasts, followed by inflammatory cells such as macrophages, lymphocytes and plasma cells, some of them invented the comet-like cells, myofibroblasts, and other photoreceptors. The basic components of vascular regeneration VEC and pericytes. CNV membrane of the invention includes discussion VEC, Zhou cells, pigment cells, collagen fibers, fibroblasts, macrophages, leukocytes, mononuclear lymphocytes.
2.1 CNV membrane component of pathological tissue
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The discussion of the origins of infinite CNV membrane preparations, other specimens due to CNV membrane is very small, less than the same time not be able to do light and electron microscopy look only at the differences do during the election of a few electron microscope inspection. Workshop discussions also need to be a large sample.Articles from wheat net health: age-related macular degeneration choroidal neovascularization of pathologic changes of Disclaimer: This article is reproduced from the network and, if violated, please us immediately removed, and this is only personal opinion of the and webmaster network nothing. The original text of the statement and the text and content without the site confirmed, and of which all or part of this content, text authenticity, integrity, timeliness, this site does not make any warranty or undertaking, the er is for reference only, and please self-verification of relevant content.


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