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Exp Content Document Name: PTEN tumor suppressor gene on the highly metastatic mucoepidermoid carcinoma cell line biological behavior of Introduction: Purpose of exogenous wild-type PTEN tumor suppressor gene on human high-metastatic mucoepidermoid carcinoma cell line M3SP2 in vitro adhesion, migration and invasion properties. Methods liposome method PTEN tumor suppressor gene into M3SP2 cells (M3SP2-PTEN cells), empty vector transfected cell line as control (M3SP2-pBp cells), respectively tetrazolium blue ( MTT) assay, cell migration and cell invasion test test measured M3SP2-PTEN cells and M3SP2-pBp cells in vitro adhesion, migration and invasion. Results M3SP2-PTEN cells Metrigel and reduce the number of Fn adhesion substrate, compared with the control group there was significant difference (P Document Name PTEN tumor suppressor gene on the highly metastatic mucoepidermoid carcinoma cell line biological behavior of Article Name
English (English) Translation Effects of the Exogenous PTEN Tumor Suppressor Gene on Adhesion, Migration and Invasion of the Highly Metastatic Mucoepidermoid Carcinoma Cell Line; of Bin; Wu JZ; Stuart Town, strong; LIU Bin; WU Jun-zheng; SITU Zhen-qiang . (Deptartment of Oral Biology; College of Stomatology; Fourth Military Medical University; Xi an 710032; China);
Agencies School of Stomatology, Fourth Military Medical University, Department of Oral Biology; Fourth Military Medical University, Department of Oral Biology School of Stomat


ology, Xi'an; Document
Article From Shanghai Institute of Metallurgy; Materials Physics and Chemistry (Professional) PhD thesis in 2000 Keywords PTEN tumor suppressor gene; mucoepidermoid carcinoma; adhesion; migration; invasion; KeywordsPTEN tumor suppressor gene; Mucoepidermoid carcinoma; Adhesion; Migration; Invasion;
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Submandibular gland mucoepidermoid carcinoma in the palate who could undermine the hard palate. Tissue origin of the disease: the main component of the tumor cells and epidermal mucus-like cells. Mucoepidermoid carcinoma derived from glandular epithelial cells. Clinical manifestations 1. Symptoms: their clinical differentiation are closely related. Well differentiated type, the majority, usually painless lump, growth slower, longer course. Tumor volume sizes, borders clearly more rigid quality, activity, the surface smooth or nodular. For cystic, can also solid. Occurred in the palate or the retromolar who showed mass in the submucosal pale blue or dark purple, mucosal smooth, soft texture, a small amount of bloody purple needle can be removed from the liquid. 2. Parts: a maximum in the parotid gland, accounting for about 70%. Small salivary glands are common in the palate, other parts, such as the retromolar area, cheek, upper lip, lower lip and other parts are rarely occurred. Can occur at any age, more common 30 to 50 years of age, more women than men, about 1.5:1. Poorly differentiated type of tumor growth and rapid, often accompanied by pain. Boundary is not clear, was diffuse, with the surrounding tissue adhesions, ulceration and secondary infection can form a prolonged unhealed ulcer surface, and a yellow sticky secretions. Sometimes form a salivary fistula. Those occurring in the parotid gland, involving the facial nerve can occur when the symptoms of facial nerve paralysis and hemifacial spasm symptoms. Occurred in the palate who could undermine the hard palate. 3. Complication: those occurring in the parotid gland, involving the facial nerve can occur when the symptoms of facial nerve paralysis and hemifacial spasm symptoms. Occurred in the palate who could undermine the hard palate. Medical Technology Check 1.CT examination showed the tumor boundary is not clear, parotid glands damaged or squeezed shift. 2. The surgery confirmed only after frozen section examination. Those in the parotid gland, parotid gland imaging visible erosion damage, catheter defect or interruption occurs distal catheter filling partially or totally, the wall is not smooth, the branch duct damage may also occur, such as malignant tumor lipiodol leakage performance. CT examination showed the tumor boundary is not clear, the parotid glands damaged or squeezed shift. Gross morphology: well-differentiated tumors were similar and mixed, were round, smaller tumor size, diameter, mostly 2 ~ 3cm, 5cm and some have more than a few capsule, but most are incomplete, or even no capsule. Profiles are pale or light pink, occasionally leaf, can have varying amounts of the small capsule, containing mucus. Poorly differentiated by a complete lack of capsule, ill-defined, invading into adjacent tissues. White cut surface, regardless of leaf, homogeneous, may have a small capsule, and scattered small focal translucent. microscopy: is a mucus-like cells, epidermoid cells and intermediate cells. Mucinous goblet cells were mature or cylindrical, transparent cytoplasm, nucleus in the base; differentiation of immature, like cancer cells, the cytoplasm containing mucus, carmine staining. , Epidermal-like cells similar to the oral mucosa of stratified squamous epithelium, intercellular bridges can be seen, occasionally keratosis. Intermediate cells were cuboidal, smaller, same size, little cytoplasm, similar to the basal epithelial cells. Intermediate cells and epidermal cells to the mucus-like cells to develop. Were well differentiated, mucus-like cells and epidermal cells are more small intermediate cells, tumor cells can form irregular flakes, but often the formation of cysts of varying sizes, wall lining common mucous cells. Mucus-like cells covering the skin-like cells can also be mixed in between the epidermal cells. Large cysts may have broke into the nipple, a red dye cavity mucus. Poorly differentiated, primarily for the epidermoid cells and intermediate cells, and less mucus-like cells, tumor cells become apparent, showing nuclear division, more substantive epithelial clumps, small cysts, and can see the tumor to the surrounding tissue invasion. biology: Changcheng invasive growth, a higher recurrence rate may be lymph node metastasis, metastasis to the bone, brain, lungs are small, but well differentiated may have complete capsule, less recurrence after complete resection. Diagnosis is based on the general subject only after surgical frozen section diagnosis. Those in the parotid gland, parotid gland imaging visible erosion damage, catheter defect or interruption occurs distal catheter filling partially or totally, the wall is not smooth, the branch duct damage may also occur, such as malignant tumor lipiodol leakage performance. CT examination showed the tumor boundary is not clear, the parotid glands damaged or squeezed shift. Occurred in the differential diagnosis of parotid gland, should be with the parotid gland tumor, mumps, parotid gland cancer, phase identification. Principle of treatment of the disease the treatment of primary tumor was mainly regional radical resection. To prevent recurrence, surgery should be 1cm away from the tumor within the normal tissues outside the tumor. Parotid well-differentiated mucoepidermoid carcinoma of the first surgical treatment, regardless of stage of disease to the general use of reserves of the facial nerve in parotid gland Excision; poorly differentiated type of invasive facial nerve more opportunities, such as facial nerve involvement, should be performed at the expense of the facial nerve in parotid gland totally removed surgery, if the length of the facial nerve and large, you can do after the removal of a nerve graft nerve. In mucoepidermoid carcinoma of submandibular gland, submandibular triangle dissection should be performed. Occurred in the palate, should be part of the maxillectomy. If the tumor has violated the surrounding tissue, should be extended resection. Regional lymph node metastasis of the lesion was low, poorly differentiated type than could be considered selective neck dissection, generally do not do well differentiated by selective neck dissection. Perzik so stressed that only when the primary tumor in the resection of cervical lymph node metastases identified only do neck dissection. The disease is not sensitive to the radiation therapy, but the poorly differentiated type of surgery with the use of radiation therapy may increase efficacy or reduce recurrence. Prognosis prognosis is good. Bhaskar reported 144 cases of recurrence were 12.5%, 5-year survival rate was 88.5%. Tumor cell differentiation and prognosis. Kosenfeld report of 5 well-differentiated type, 10-year survival rate was 100%, while the poorly differentiated type of 5, 10-year survival rates were 39% and 11%. Spiro and other reports 367 cases, including well-differentiated type 5, 10 and 15 year survival rates were 92%, 90% and 82%; poorly differentiated type were 49%, 42% and 33%. Primary in the parotid gland and small compared to those occurred in the submandibular salivary gland were as good. Guo-Chu Lin and other reports 189 cases, the local recurrence rate was 13.57%, 5, 10, 15 year survival rates were 91.66%, 89.87%, 66.61%. 1


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