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Abstract: Objective: To investigate the dendritic cells (Dendritic cell, DC) in aplastic anemia (AA) pathogenesis, especially for the abnormal activation of the activation of lymphocytes. Methods: The direct immunofluorescence flow cytometry analysis of bone marrow in patients with I-AA ratio of DC; in vitro by recombinant human granulocyte colony stimulating factor (rhGM-CSF) and interleukin 4 (IL-4) induced peripheral blood mononuclear cells into DC, DC phenotype by flow cytometry and observed by the purification of human umbilical cord blood CD34 ^ + cells antigen, the soluble human CD40 ligand (rhsCD40L) stimulate autologous T cells after activation and expansion effects; real-time quantitative PCR (RQ-PCR) quantitative determination of T cells before and after the DC load TCRV antigen gene expression profile changes. Results: AA patients with bone marrow CD1a ^ + CD11c ^ + cells and CD11c ^ + CD83 ^ + double-positive cells was increased, and the CD11c ^ + CD83 ^ + double-positive mature DC-based; peripheral blood of human cord blood CD34 ^ DC load + cells against autologous lymphocytes significantly stimulate the gene expression profile and make T cells TCRV access shift occurred. Conclusion: DC oligoclonal lymphocyte proliferation in AA play an important role, but what kind of antigen recognition still further study. Number of pages: 5 Page Range :174-178 Key words: Dendritic cells in aplastic anemia T cell receptor V gene real-time quantitative PCR library subje


ct classification: R322.2 [medicine, HEALTH "Basic Medi Human Form Sci Human Ana blood-forming organs, lymphatic system] Related Articles: Topics related references (12) citations (1) coupling the literature (3)
haozi1989 Grade: Pvt respondents: haozi1989 Posted : 05:46 Points: 5808 Welcome Qianlong doctor
1, note that patterns of life, to maintain ease of mind, work and rest, exercise, develop good health habits, morning and evening brushing, as little or less public places to avoid contracting the disease; ban violent campaign to prevent accidents led to bleeding.
2, for severe acute type and absolute bed rest is required; chronic aplastic anemia patients without spontaneous bleeding, hemoglobin has risen to those who can tolerate general activity, may participate in some physical activity without undue restrictions, such as light can participate in family manual, and can participate as appropriate in the general sports activities such as walking, tai chi, massage, the strong body, the hematopoietic function recovery. Aplastic anemia {note}
3, directed by your doctor eat: avoid spicy spicy food (raw onions, ginger, garlic, pepper, etc.), seafood, lamb, dog and other hot foods, avoid alcohol, avoid cold greasy; given high-protein, high vitamins, digestible food such as lean meat, eggs, milk, chicken, pork ribs soup, liver, fresh vegetables and fruits, eat dates, Woodcrest Hill, peanuts, walnuts, lotus root and blood to stop bleeding; For those who give no bleeding tendency half residue liquid diet, eating less barbed, bone food, to prevent bleeding and infection caused by stab wounds. Aplastic anemia {note}
4, note that in patients with bleeding tendency, such as skin and mucous membrane bleeding, epistaxis, gingival bleeding, retinal bleeding, and bleeding to give symptomatic treatment; presence of GI bleeding or the risk of intracranial hemorrhage should be immediately reported to a doctor at the same time y use of rescue medication and a variety of materials to assist in the rescue.
5, to keep wards clean and disinfect the air every day, leukopenia protective isolation should be lines to reduce the infection.
6, note that oral hygiene and anal hygiene. Adhere to the meal, before going to bed mouthwash, to prevent oropharyngeal ulcers, commonly used mouthwash with saline solution, boric acid, 1% hydrogen peroxide, sodium bicarbonate solution, etc.; adhere to it with 1 / 5000 potassium permanganate solution to sit bath to prevent the occurrence of necrotic ulcers around the anus caused by sepsis.
7, skin, mucous membrane bleeding keeping a wide range of skin and mucous membrane integrity to prevent infection, high fever sweat are changing in time, to avoid catching a cold.
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Exp Content Document Name: Tcf-1 gene in patients with aplastic anemia bone marrow CD4 ~ T cells Introduction: Objective To investigate the Tef-1 gene in aplastic anemia (AA) in patients with CD4 ~ T cells in the bone marrow expression. Methods Mini-MACS magnetic bead separation system isolated from the bone marrow CD4 ~ T cells, with half given the RT-PCR detected Tef-1 mRNA in patients with aplastic anemia and normal bone marrow CD4 ~ T cells. Results Tef-1 mRNA in the bone marrow of patients with AA CD4 ~ T cells was significantly higher than normal (P <0. 01), and SAA in patients with bone marrow CD4 ~ T cells was significantly higher than non-heavy aplastic anemia patients (P <0. 01). Conclusion Compared with normal, Tef-1 gene in patients with aplastic anemia bone marrow CD4 ~ T cells upregulated, and in severe aplastic anemia in patients with CD4 ~ T cells in the bone marrow express higher levels, suggesting Tef-1 gene expression level of may be related to the proliferation of bone marrow CD4 ~ T cells related to the degree of activation.
Objective To explore the expression level of Tcf-1 in the bone marrow CD4-T cells of patients with aplastic anemia.Methods The bone marrow CD4 ~ T cells were separated with magnetic bead sorting technique (Mini-MACS). The expression level of Tcf -1 mRNA was detected by semi-quantitative reverse-transcription poly-merase chain reaction.Results The expression level of Tcf-1 mRNA in the bone marrow CD4 ~ T cells of patients with aplastic anemia was obviously higher than that of normal group (P <0.01). The expressio . Document Name Tcf-1 gene in patients with aplastic anemia bone marrow CD4 ~ T cells Article Name
English (English) Translation Expression of Tcf-1 in the Bone Marrow CD4 ~ T Cells of Patients with Aplastic Anemia; of Miao Cheng; Wen-Li; Sun Hanying; Zhou Jianfeng; Fujin Rong; Lori; ZHENG Miao LIU Wen-li SUN Han- ying (Department of Hematology; Tongji Hospital; Tongji Medical College; Huazhong Science and Technology University; Wuhan 430030);
Agencies Huazhong University of Science Tongji Hospital, Tongji Medical College, Department of Hematology; Tongji Medical College, Huazhong University of Science and Technology Department of Hematology, Tongji Hospital, Wuhan; Wuhan; Document
Article From Shanghai Institute of Metallurgy; Materials Physics and Chemistry (Professional) PhD thesis in 2000 Keywords Tef-1; aplastic anemia; CD4 ~ T cells; KeywordsTcf-1; aplastic anemia; CD4 ~ T cell;


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