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Dilated cardiomyopathy (IDC), is a serious heart disease, so far, the cause of dilated cardiomyopathy and pathogenesis is still unclear, many studies found that patients with dilated cardiomyopathy have a family history of genetic tendency, and its incidence and immune imbalance. Particularly severe dilated cardiomyopathy, protraction, causing great harm, as early diagnosis and symptomatic treatment is particularly important. The present study indicate that the pathogenesis of dilated cardiomyopathy genetic abnormalities may be the primary immune mechanisms of dilated cardiomyopathy may be an autoimmune disease. Interferon (IFN-r) in the body's inflammatory immune response and play an important role in the process may be related to the occurrence of dilated cardiomyopathy is closely related to the development and pathological processes.
Many studies have found that interferon in the body's inflammatory immune response and play an important role in the process may be related to the occurrence of dilated cardiomyopathy is closely related to the development and pathological processes. Interferon is interferon gene expression product, the gene has the polymorphism 874 points (SNP), and interferon 874T is a product of the high expression of phenotype, interferon gene 874 point type and the expression of interferon disease susceptibility. Interferon is not currently see with the genotype associated with dilated cardiomyopathy reported. We examined the interferon gene polymorphism 874 changes to interferon immune and genetic factors of dilated cardiomyopathy in the pathogenesis of the possible role and clinical significance.1, the subjects
Total patients with dilated cardiomyopathy 3l patients (by history, physical examination, the physical diagnosis and histopathological diagnosis, etc.), diagnosis of dilated cardiomyopathy cases in 1980, according to the criteria established by the World Health Organization in all cases of cadres from the provincial clinics and nursing homes Screening hospital patients identified, are in unrelated Han individuals in the north, l9 male, female l2 cases, 35 cases of normal control group, both the North Han, unrelated healthy blood donors, blood sampling all subjects 5ml, EDTA anticoagulant.
2.DNA blood extraction mining 5ml, 5% EDTA anticoagulant, using the method described Res.se extract genomic DNA.Detection of interferon gene promoter primers were 874 Lai Mude Biotechnology Co., Ltd. from the United States to provide, based on a pair of specific primers terminated primers: P1: 5 for a GTGGAGGCTGAACCCCGTCCT a 3: P2: 5 a 3 a GTGGAGGCTGAACCCCGTCCT interferon promoter Gene 874 points and the specificity of gene coding sequence in Table Table 1 interferon gene of 874 points of type polymorphismApplication of the United States produced by PCR Instrument PE (4800) and related reagents, PCR reaction volume in 50 l, 94 denaturation 5min, then 94 , 30s; 60 annealing, 50s; 72 extension, 40s; a total of 30 cycles, extension stimulation were characterized by conventional gel electrophoresis in 2% agarose ,15-20min, UV light observation.
5. Application of SPSS statistical analysis system software.1. Standard interferon DNAPCR-SSP typing results of standard interferon DNA typing, see the 250bp fragment and with a positive band appears to be positive in the control. This article uses the kit to detect interferon template DNA positive, the results of each sample were banding, suggesting that the gene fragment amplification is required for success, shown in Figure
2. Interferon DNA samples, PCR-SSP typing results, type of interferon gene polymorphism in normal populations and dilated cardiomyopathy in the distribution in Table Dilated cardiomyopathy interferon 874T allele frequency was significantly higher than PInterferon (IFN-r) is a low molecular weight glycoprotein, is one of important cytokines, mainly by lymphocytes, monocytes and macrophages released and involved in human inflammatory and immune response. Synthesis and synthesis of interferon is controlled by genes. The synthesis of individual differences, and some can be a difference of more than a few times. The main reason may be differences in cytokine gene regulatory regions or the promoter region polymorphism. In vitro tests showed that the cells were stimulated independent of each cytokine secretion vary greatly. In addition, a synthesis of some of interferon, whereas the other is low the synthesis of interferon, this difference is not the result of regulating the immune system, but the interferon variant genotype polymorphism. Individual differences in the synthesis of interferon great now that the TNF gene promoter region or regulatory regions of different alleles can also be said that direct control of the polymorphisms of interferon synthesis.
According to genotype and serum cytokine levels of cytokines in the clinical relationship between the synthesis of cytokines have been divided into high, medium and low indicators and use of three different cytokine levels and clinical disease. Has been proved that many diseases and pathological processes related to the corresponding cytokines. Interferon is an important human cytokines. In autoimmune diseases, play an important role. Polymorphic interferon, interferon gene polymorphism upstream of 874 point mutation, the first 874 points from A into T base due. According to genotype and serum cytokine levels of cytokines in the clinical relationship between the synthesis of cytokines have been divided into high, medium and low indicators and use of three different cytokine levels and clinical disease. Has been proved that many diseases and pathological processes related to the corresponding cytokines. Interferon is an important human cytokines. In autoimmune diseases, play an important role. The results showed dilated cardiomyopathy interferon (874T) allele frequency was 32.26%, while the control group was 11.43%, dilated cardiomyopathy interferon (874T)) allele frequency was significantly higher than control group, compared with the normal healthy group, P
Our study further elucidate the pathogenesis of dilated cardiomyopathy mechanism for the clinical treatment of disease prevention and control of the disease and provide a theoretical foundation based on response. In addition, the study of other autoimmune diseases provides useful clues. This study shows from one side of interferon (874T) of the genotype variability of dilated cardiomyopathy play an important role, suggesting that: when a person of interferon (874) gene is a T, on the susceptibility to dilated cardiomyopathy , and its interferon (874T) secretion is also high, indicating that interferon (874T) locus genotype can be used as dilated cardiomyopathy to determine susceptibility and disease indicators. For interferon (874T) mutation sites, and individual susceptibility and pathogenesis of dilated cardiomyopathy causal relationship needs further study.


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